ANALYTE DETECTION

Analyte Sensors, Methods for Preparing and Using Such Sensors,
and Methods Of Detecting Analyte Activity

INVENTOR

Jenny Yang
Patent Status: Patent Pending
GSU case no 2011-06

INTRODUCTION

Quantitative, real time and fast detection of metal ions is needed to understand their roles in normal physiological functioning and bodily disorders. Current metal ion sensors need improvement in specificity, accuracy and ease of use.

TECHNOLOGY

Georgia State University researchers have devised analyte sensors for quantitative measurement of metal ions, which comprise molecular recognition motifs linked to or integrated within optically active fluorescent host proteins. These molecular recognition motifs consist of particular nucleotide sequences and bind to metal ion analytes. As a result of binding, the analyte-protein assembly produces a detectable change in fluorescence. The operation involves inserting the sensor in a solution containing metal ions, providing a source of magnetic resonance for excitation, followed by measurement of fluorescence signal.

CONTACT US
Chester A. Bisbee
Associate Vice President and Director
Office of Technology Licensing and Commercialization
217 Dalberg Hall
404-413-3507
cbisbee@gsu.edu

 

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Analyte Sensors, Methods for Preparing and Using Such Sensors, and Methods Of Detecting Analyte Activity

APPLICATIONS

  • Detection and measurement of metal ions such as Ca2+, Pb2+, Tb3+, Ln3+ and Gd3+
  • Suitable for in vitro and in vivo
  • Analysis of biological, environmental and plant samples containing metal ions
ADVANTAGES

  • Small, sensitive and quantitative sensors
  • Stable at temperature greater than 30 ºC
  • Enhanced fluorescent and optical properties
  • Usable inside cells. Can be expressed inside a host using a vector system
  • Can be targeted to different subcellular compartments