Induction and Stabilization of Enzymatic Activity in Microorganisms


Inventor: George Pierce
IP Status: Patent pending
GSU case no.: 2006-06


There is considerable interest in using biological methods to remove and remediate hazardous industrial chemical waste. Bio-detoxifying microorganisms capable of utilizing their enzymes to break down the hazardous chemicals have been successful in industrial use. However, there is a need for methods with enhanced microbial activity and improved enzymatic stability. Another concern is that induction of several enzymes has traditionally required using hazardous materials. Less toxic and environment friendly methods are needed to employ microorganisms for biodegrading purposes.


Georgia State University has developed a technology of growing microorganisms for specifically producing enzymes with inducible and stabilized activity. The induction is done through the use of specific growth media comprising amide containing amino acids and their derivatives. This technology has been demonstrated in Rhodococcus bacteria producing nitrile hydratase, amidase and asparaginase-I enzymes. The enzymes break down toxic materials such as nitriles to relatively harmless amides and acids.

Chester A. Bisbee
Associate Vice President and Director
Office of Technology Licensing and Commercialization
217 Dalberg Hall
[email protected]


PDFInduction and Stabilization of
Enzymatic Activity in Microorganisms


  • Microbial detoxification of harmful compositions containing nitrile compounds
  • Cultivating and harvesting a variety of microorganisms capable of producing certain enzymes
  • Induction, stabilization of nitrile hydratase, amidase and asparaginase-I in various microbes
  • Simultaneous induction of enzymes leading to a one-step treatment process

  • Bio-detoxifying catalysts capable of maintaining commercially useful enzymatic activity
  • Enzyme induction and stabilization using non-hazardous media additives, such as amino acids
  • Improved enzyme stability to facilitate immobilization  Enhanced enzymatic activity